For the
detection of clinically relevant Candida species we can
use samples such as mouth washes, swab-collected material,
sputum, feces and urine samples.
Samples should be collected in sterile containers and shipped
leak- and break-proof. Fast (i.e. overnight) shipping to our
laboratory is essential.
Please follow the recommendations (according to Prof. Dr. Dr. H.
Rieth: Mykologische Diagnostik, GIT Verlag, Darmstadt) in sampling as listed below:
- sampling without prior
disinfection
- collect outflow with a sterile swab
- send swab in a sterile capped tube (e.g.
Falcon tube)
- if necessary, cut off swab head
Oral candidiasis and candidiasis of infant
skin
- scrape off layers of infection with a
sterile spatula or swab
- collect material in a sterile, capped tube
- if necessary, cut off swab head
Collection of sputum for mycological analysis
- before sampling: Rinse the mouth twice with
an antifungal mouth wash fluid
- bring up sputum by clearing the throat and
coughing
- collect sputum in a sterile container
thereby avoiding the use of the tongue and lips
Collection of urine
- when possible collect by punction
- otherwise collect midstream urine in a
sterile container
- centrifugate the urine in a sterile capped
centrifuge tube (e.g. Falcon tube)
- discard the supernatant
- send in the wet pellet in the centrifuge
tube
Collection of feces samples
As fungi are not equally distributed in the feces, but grow in
colonies and „nests", it is important to take several
smaller samples (at least 5) at different positions. Collect
about a hazelnut sized sample in a sterile capped tube with a
sterile spatula.
Yeast infection in general is located in the
small intestine. The detection of clinically relevant Candida
species in faeces samples therefore gives an indication of an
intestinal Candidiasis. If possible, faeces samples from at
least three subsequent days should be collected.
Microbiological identification
Samples are spread on Oxoid-Chromogen-Candida-Selective
Agarplates (Oxoid GmbH, Wesel, Germany). In a Candida
mixed infection the following species can be determined:
- Candida albicans
- Candida glabrata
- Candida krusei
- Candida tropicalis
- Candida parapsilosis
Molecular biological identification
From pre-selected yeast colonies the genomic DNA is isolated. By
PCR amplification of the DNA, a part of the 18S ribosomal RNA
gene is amplified and isolated. Sequence analysis of the
fragment and comparison with reference sequences identifies the
species (Ribotyping).
By ribotyping, rare and poorly cultivated
species can also be identified.
The identification of Candida species
usually takes 3-5 working days after receipt of the sample
material. Please
contact us
for an estimate for the molecular biological typing (Ribotyping)
of Candida species |
|
Please
contact us
for an estimate for the molecular biological typing (Ribotyping)
of Candida species. |
| The
identification of Candida species usually takes 3-5
working days after receipt of the sample material. |
More
information for physicians and patients under the
following links:
www.candidapage.com (Several links to sites
specialized on Candida).
www.health-truth.com
(The Biamonte Center for Clinical Nutrition,
Information
for affected patients)
www.candida.de
(Deutsche Candida Hilfe e.V., Hamburg, Information for
affected
patients in Germany). |
|
